Vertical Gel Units


The TV Range

The Scie-Plas 'TV Range' vertical gel electrophoresis units offer the ultimate standards in innovative design and manufacture acquired during our 20+ year existence.

The 'TV Range' vertical gel electrophoresis units are available in a range of different sizes for applications ranging from routine SDS-PAGE mini-gels to large format nucleic acid sequencing and single-strand conformational polymorphism studies (SSCP). As with our 'Green Range' horizontal gel units, each 'TV Range' vertical gel electrophoresis unit incorporates the many design and safety features recommended to us over the years by scientists, either from within our in-house product development team or laboratories worldwide. Accordingly, each unit is manufactured and finished to the highest specification, versatile, easy to use and fully compliant with European safety regulations.

The 'TV Range' vertical gel electrophoresis units are availabe in a range of different formats:

The TV50 single-plate 10 x 10cm (W x H) mini-gel unit is a good, cost-effective option for routine, low throughput separation of proteins and nucleic acids in self-cast or commercially available precast gels.

The TV100 twin-plate 10 x 10cm (W x H) mini-gel unit is, historically, our best selling vertical gel electrophoresis unit which is designed for all standard polyacrylamide gel electrophoresis (PAGE) applications. This unit is ideal for use with commercially available precast gels or gels cast using our new-style GRM and cam-pin casting base. Here, gel cassettes are placed within the GRM and then pulled tightly onto silicone seals by cam pins located in the casting base. Glass plate stops situated within the GRM immediately above the glass plates serve as an immovable barrier, locking the plates firmly into position on the casting base as the cam pins are turned clockwise through 120°. The gel can then be poured in total confidence that it will be leak-free. Each TV100 unit is also available as a cooled tank option, and can be adapted, either as a stand-alone unit or as part of a fully integrated system, with specific modules for electroblotting and 2-D electrophoresis. The TV200 twin-plate 20.5 x 10cm (W x H) wide format mini-gel electrophoresis unit allows more samples to be run within the same gel, eliminating the problems that can be associated with comparing samples from 2 separate mini-gels.

The TV200 offers the same benefits as the TV100, including a GRM with glass plate stops and cam-pin casting system, in addition to a cooled-tank and modular electroblotting and capillary gel 2-D electrophoresis options.

The TV400 twin-plate 20.5 x 20cm (W x H) maxi-gel unit offers all the benefits of the TV100 and 200 units, but in a much larger gel size and with greater sample throughput. The TV400-GRM also includes glass plate stops which act in tandem with the cam-pin casting base to provide leak-proof casting, whereas an optional cooled tank format can be connected either to the laboratory water supply or external chiller to maintain the cooling necessary for high quality resolution. Specific modules again allow the TV400 to be adapted either as stand-alone electroblotting and 2-D electrophoresis units or as a fully integrated system.

The TVS1400 single-plate 33 x 41cm (W x H) large format vertical gel unit allows more samples to be processed during each electrophoretic separation.

CASTING WITH NEW-STYLE GRM

1. Place each plain glass plate on a level bench surface, followed by the spacers and then the notched glass plate

2. Insert the glass plate cassette into the gel-running module (GRM), placed on its side on the bench, and lightly tighten the screws - repeat for the other cassette by turning the GRM on its other side

3. If not using plain glass plates with bonded spacers, get a spacer aligner to align the spacers flush with the vertical edges of the plain glass plates before tightening the screws within the GRM

4. Run your forefinger along the bottom edge of the glass plates to ensure that they are flush with bottom edge of each spacer - if not, repeat step 3

5. Lower the assembled GRM onto the casting base with the cam pins pointing downwards into the bench surface

6. Turn the cam pins through 120° to secure the GRM onto the casting base - as you turn the cam pins you will experience increased resistance

7. Carefully insert the appropriate comb allowing the gel to set for at least 30 minutes

8. After carefully removing the combs, release the GRM by turning the cam pins in the opposite direction on the casting base

9. Place the GRM into the gel tank, ensuring that there is sufficient buffer to cover the bottom of the glass plates

10. Fill the inner buffer chamber within the GRM so that it just covers the top of the gel - the gel is now ready to be loaded



11. Replace the lid and insert the power cables into the 8, making sure that it is first switched off - the unit is now ready for electrophoresis

Benefits
Robust acrylic tank construction
Safety lid with integral power leads
Shrouded 4 and 2mm power output connectors universally compatible with modern-day low, medium and high voltage power supplies
Long-life, 99.99% pure platinum electrodes
Gold-plated terminals with corrosionfree stainless steel washers
Offset asymmetric electrodes
A versatile, time-saving gel-running module (GRM) which supports both gelcasting and electrophoresis
Durable high-impact polystyrene (HIPS) and melinex combs which are colour-coded, corresponding to 0.25, 0.35, 0.75, 1, 1.5 and 2mm thicknesses
Electroblotting and 2-D capillary gel modules available for use within the same tank either as part of a fully integrated system or as a stand-alone u
Benefits


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