The IEF-SYS Dedicated Isoelectric Focusing Unit


The recently redesigned Scie-Plas IEF-SYS unit has been optimised for isoelectric focusing applications using IPG BlueStrips and SERVALYT™ PRECOTES™ and PreNet™ IEF gels, also available through Scie-Plas.


1. Set the cooling temperature of the external chiller, connected to the cooling plate by quick-fit tubing, to 4°C at least 10 minutes before using the unit.

2. Place the gel directly on top of the area covered by Bayol F or kerosene and, by using a pipette or electrophoresis roller (42991.01), gently disperse any bubbles underneath the gel.

3. Immerse each electrode wick (42988.01) in its respective electrode solution (Scie-Plas recommends 100µl of electrode solution per cm of wick), depending on whether it is the anode (42984.03) or the cathode (42986.03). The length of the electrode wicks should correspond to the width of the precast gel.

4. Position the electrode wicks at either end of the IEF gel, at least 5mm inside each edge, so that they are aligned parallel with the red and black strips at either end of the gel. The distance between each
electrode should be 10cm for SERVALYT™ PRECOTES™ IEF gels.

5. Place a clean, washed applicator strip (42915.01) in the middle of the gel.

6. Load 10µl of protein sample into each well with a pipette, reserving at least one well for a 10µl-aliquot of SERVA Liquid Mix IEF Markers 3-10 (39212.01).

7. After loading the gel, carefully lower the glass electrode weight over the anode and cathode electrodes, so that they remain in contact with the electrode wicks and gel during the application.

8. Perform IEF according to the recommended running conditions in Technical Specification. Once IEF is complete, the SERVALYT™ PRECOTES™ IEF gel is ready for staining. Protein samples in a SERVALYT™ PRECOTES™ Wide Range pH 3-10 IEF gel following IEF with the IEF-SYS unit and fixation and staining with SERVA Violet 17.



Benefits
Ceramic cooling plate27 x 27cm (W x L) - can support either:
  • One 245 x 125mm or two 125 x 125mm SERVALYT™ PRECOTES™ IEF gels;
  • Or up to a maximum of 30 IPG BlueStrips, each 3mm wide and up to 24cm in length
Ceramic cooling plate can also be connected to an external chiller to maintain IEF at:
  • 4°C for SERVALYT™ PRECOTES™ and PreNet™ IEF gels
  • 20°C for IPG BlueStrips
Acrylic electrode frame sits directly on the ceramic cooling plate for IEF with SERVALYT™ PRECOTES™ and PreNet™ IEF gels
Glass electrode frame sits flush with the ceramic cooling plate for optimal cooling efficiency and minimises mess caused by oil application during IEF with IPG strips
Positive and negative electrodes made from clear acrylic clip neatly within the acrylic or glass electrode frame, allowing the voltage gradient to be fine-tuned along the entire length of each IPG strip
Glass electrode weight ensures that the electrodes remain in complete contact with the positive and negative ends of each IPG strip or IEF gel during IEF
2mm shrouded power output connectors compatible with high voltage power supplies
IEF tank or cooling plate can also be used for wick-based horizontal gel electrophoresis


Ordering Information

Complete System

Flat-bed isoelectric focusing unit includes running tank, lid, 1 x ceramic cooling plate, 1 x glass electrode frame, 1 x electrode frame, 1 x anode electrode, 1 x cathode electrode, 1 x glass electrode weight, 2 x quick-fit tubes and 2 x 2mm power cablesIEF-SYS1.506,00 €

Replacement Parts & Accessories

1 x ceramic cooling plate for IEF-SYSIEF-CP332,00 €Add to your cart
1 x glass electrode frame for IEF-SYSIEF-GF285,00 €
1 x electrode frame for IEF-SYSIEF-EF239,00 €Add to your cart
1 x cathode electrode for IEF-SYSIEF-CE189,00 €
1 x anode electrode for IEF-SYSIEF-AE189,00 €Add to your cart
1 x glass electrode weight for IEF-SYSIEF-GS60,00 €
2 x quick-fit tubes for cooling plate for IEF-SYSTCS-CC63,00 €Add to your cart
2 x 1 metre power leads with shrouded 2mm power output connectorsCABLE-253,00 €
Platinum WirePT-0.2100CM114,00 €Add to your cart

Technical Specification

IEF-SYS Unit

see ordering details
Maximum Sample Capacity30 x IPG strips
Unit dimensions (WxDxH) inc. cooling coil46 x 41 x 11.5cm
Inner Tank Dimensions (WxDxH)37 x 31 x 8.5cm
Glass plate active dimensions (WxLxD)27 x 27 x 0.5cm
Electrode frame active dimensions (WxLxH)27 x 27 x 1cm
Cooling plate dimensions (WxLxD)27 x 27 x 3cm
Glass electrode weight active dimensions (W x L x H)20 x 26.5 x 1cm
Adjustable electrodes active dimensions (W x L x H)10 x 265 x 13mm
Recommended temperature for cooling plate during IEF with IPG strips20°C
Recommended temperature for cooling plate during IEF with IEF gels
Power Output Connectors (diameter)Shrouded, 2mm
Recommended Running Conditions for PRECOTES™ IEF gels
Voltage Step 1Voltage (V) 300
Time (min) 20
Volt-hours 100
Voltage Step 2Voltage (V) 600
Time (min) 20
Volt-hours 200
Voltage Step 3Voltage (V) 1000
Time (min) 30
Volt-hours 500
Voltage Step 4Voltage (V) 1200
Time (min) 30
Volt-hours 600
Voltage Step 5Voltage (V) 1500
Time (min) 30
Volt-hours 750
Voltage Step 6 end of runVoltage (V) 2000
Time (min) 60
Volt-hours 2000
Recommended Running Conditions for IEF of 7cm IPG strips
Voltage Step 1Voltage (V) 150
Time (h) 0,5
Volt-hours 75
Voltage Step 2Voltage (V) 300
Time (h) 0,5
Volt-hours 150
Voltage Step 3Voltage (V) 600
Time (h) 0,5
Volt-hours 300
Voltage Step 4Voltage (V) 1500
Time (h) 0,5
Volt-hours 750
Voltage Step 5Voltage (V) 300
Time (h) 2,5
Volt-hours 7500
Voltage Step 6 end of runVoltage (V) 330
Time (h) <20
Volt-hours -
Recommended Running Conditions for IEF of 18cm IPG strips
Voltage Step 1Voltage (V) 300
Time (h) 1
Volt-hours 300
Voltage Step 2Voltage (V) 600
Time (h) 1
Volt-hours 600
Voltage Step 3Voltage (V) 1500
Time (h) 1
Volt-hours 1500
Voltage Step 4Voltage (V) 3000
Time (h) 12,5
Volt-hours 37500
Voltage Step 5 end of runVoltage (V) 330
Time (h) <20
Volt-hours -
Snap-lock Connectors for Cooling Coil
Inner Diameter10mm
Outer Diameter12mm
Quick-fit Tubing
Inner Diameter10mm
Outer Diameter12mm
Benefits
Ordering Information
Technical Specifications


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Downloads
PDF-FileIEF-SYS.pdf
0.12 MB


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