Professionelle Corona Test Kits von AlphaMetrix

COMET Single Cell Gel Electrophoresis Systems


The COMET Single Cell Gel Electrophoresis Systems are designed specifically for single cell gel electrophoresis (SCGE). Comet Assays are used to detect and quantify DNA damage and repair within individual cells in genetic toxicology and carcinogenesis studies.

Background - The Comet Assay/SCGE was pioneered by Östling and Johansson (1) in 1984 as a neutral pH assay to quantify double-stranded DNA breakages (DSBs) in single cells exposed to ϒ irradiation. Singh
et al. (2) then modified the assay in 1988 to a more versatile and sensitive alkaline method to measure both single- (SSBs) and double-stranded DNA breakages. Now, by introducing subtle changes to the assay conditions (pH/temperature), the Comet Assay can be tailored to analyse specific DNA lesions and repair processes.

Overview - The Comet Assay is based on the principle that strand breakage of supercoiled duplex DNA reduces the size of the large genomic DNA molecule from which these strands are separated or stretched out by electrophoresis. The high pH of the alkaline lysis step causes denaturation, unwinding of the duplex DNA and the release of alkali labile sites as SSBs. These then become "comets" as the broken ends of the negatively charged DNA molecule migrate towards the anode during electrophoresis.

Method - Cells, previously exposed to a genotoxic insult, are suspended in low melting point agarose (LMP) and embedded within a thin agarose gel on a microscope slide. All cellular proteins are then removed by lysis in detergent solution, when the DNA is allowed to unwind in the neutal/alkaline pH conditions of the detergent. The DNA is electrophoresed and then stained with a DNA-specific fluorescent dye such as acridine orange, ethidium bromide, propidium iodide or 4',6'-diamidino-2-phenylindole (DAPI). Result - Upon staining cellular DNA ismeasured for fluorescence, usually with a microscopy imaging system. The resulting image resembles a "comet", with the cellular DNA segregating into a "head" and "tail". The head is composed of largely intact genomic DNA, while the tail comprises damaged (SSBs, DSBs) or fragmented DNA, with the fluorescence intensity and length of the tail being directly proportional to the extent of the DNA damage.

References - 1. Östling, O., and Johanson, K.J., Microelectrophoretic study of radiation-induced DNA damages in individual mammalian cells. Biochem. Biophys. Res. Commun., 123, 291-8 (1984). 2. Singh, N.P, et al., A simple technique for quantitation of low levels of DNA damage in individual cells. Exp. Cell Res., 175, 184-91

Available in 2 formats to accommodate 20 or 40 standard microscope slides respectively
Opaque ebony acrylic tank and lid provide ideal protection for techniques requiring light-sensitive stains or samples
Cooled central platform provides a convenient surface for slide preparation in addition to maintaining slide temperature during pre-incubation and electrophoresis (see below)
Cooling ports can be connected to an external chiller unit: to prevent overheating during SCGE/Comet assays, typically performed at high current settings over 300mA; and to inhibit DNA repair enzyme activity by maintaining the slide temperature at 4°C, either during preparation and mounting or preincubation and electrophoresis
Buffer recirculation ports can be connected to a peristaltic pump to maintain buffer circulation, preventing ion gradient formation
Colour-coded handles corresponding to the anode and cathode - serve as a visual aid to ensure that the slides are positioned in the correct polar orientation for the assay

Ordering Information

Complete System

Comet assay system for twenty 75 x 25mm slides, including 27.5 x 21.5cm tank and lid made in ebony acrylicCOMET-20760,00 €
Comet assay system for forty 75 x 25mm slides, including 27.5 x 37cm tank and lid made in ebony acrylicCOMET-40940,00 €Add to your cart

Replacement Parts & Accessories

2 x 1 metre power leads with shrouded 4mm power output connectorsCABLE-4please contact us
Platinum Wire - 2 x 0.2mm thick platinum electrode wirePT-0.2128,00 €Add to your cart

Technical Specification


see ordering details
Unit Dimensions (WxLxH)31x32x6.5cm
Active Tank Dimensions (WxLxH)27.5x21.5x3.5cm
Recommended Buffer Volume (ml)600ml
Buffer Recirculation Ports2
Slide Capacity (25 x 75mm; W x L)20
Recommended Running Conditions5V/cm (300mA)
Power Output Connectors (diameter)Shrouded, 4mm
Recommended Power SupplyConsort EV261


see ordering details
Unit Dimensions (WxLxH)31x47.5x6.5cm
Active Tank Dimensions (WxLxH)27.5x37x3.5cm
Recommended Buffer Volume (ml)800ml
Buffer Recirculation Ports2
Slide Capacity (25 x 75mm; W x L)40
Recommended Running Conditions5V/cm (300mA)
Power Output Connectors (diameter)Shrouded, 4mm
Recommended Power SupplyConsort EV261
Ordering Information
Technical Specifications

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