The ElectroPrep™


The ElectroPrep™ system from Harvard Apparatus is an extremely versatile patented sample preparation technology based on the principles of electrophoresis and dialysis. This ElectroPrep™ system is ideal for the rapid purification of proteins, nucleic acids, carbohydrates and other biological molecules. With a run-time of 5 to 10 minutes, ElectroPrep™ provides speed and convenience, even at the very low 5 to 10mA currents used with this system. The sample chambers are made of Teflon, a completely inert material especially suited to high sample recovery. Membranes with molecular weight cut off points (MWCOs), ranging from 100 to 300,000 Daltons, may be used in combination with different Dialysis and Link Chambers for selective elution, filtration, dialysis, fractionation and concentration of complex samples, while Union chambers enable different Dialysis Chambers to be joined together to increase sample volume.



How to select your chamber and membrane configurations?


1. Decide upon your application - e.g. electroelution, electrodialysis, electrofiltration or electroconcentration

2. Select a Dialysis Chamber able to accommodate the desired sample volume - e.g. 50, 100, 200, 500, 1000 or 1500 µl - N.B. a Dialysis Chamber can be joined to a Union to increase the sample volume

3. Choose membranes with the appropriate MWCO, depending on the technique being performed (see I to IV) and the molecular weight of the biological molecule of interest dialysed product), taking into account each membrane's suitability for use in aqueous solutions or organic solvents

  • For organic solvents, use either regenerated cellulose or polycarbonate
  • For aqueous solutions, use cellulose acetate
4. Connect the Dialysis Chamber via a
membrane and Union to another Dialysis Chamber for electrofiltration (I) or electroelution (II); a Dialysis Chamber of smaller volume can also be attached to concentrate the dialysed product (III)

5. When desalting PCR® products, membranes can be secured at each end of the Dialysis Chamber by Teflon end caps (IV)

6. Link chambers can be attached to the
Dialysis Chamber via a membrane to filter samples according to their size; multiple link chambers can be used with different membranes for sizefractionation of different dialysed products

(I) Rapid and Selective Electro-Filtration / Concentration
The sample is placed in a compartment comprising the Dialysis Chamber (1) and the union. The MWCO of membrane (b) is larger than the molecular weight of the biological molecule of interest, while the MWCO of membrane (a) is smaller. Upon application of the electric field, the biological molecule of interest will pass through membrane (b) and collect in Dialysis Chamber (2) while smaller molecules will pass through membranes (a) and (b) and Dialysis Chamber (2) as a result. This is a fast and effective method for the concentration of small samples as well as selective filtration.

(II) Gel extraction of DNA and protein by Electroelution
By using the ElectroPrep™ system as shown in the schematic diagram, proteins and DNA can be eluted from a gel slice quickly and easily with excellent recovery. By using a Union, Dialysis Chambers can be joined in any combination necessary to accommodate the size of the gel slice. Samples can be concentrated if desired, by choosing a receiving chamber with a smaller volume. The MWCO of the membranes (a and b) can also be chosen to achieve very selective filtration. Membrane (b) should have a MWCO greater than the molecular weight of the sample of interest, while membranes (a) and (c) are smaller. The same principle applies as described in (I).

(III) Selective Electro-Filtration / Concentration / Separation Based on Different Charges of Biological Molecules
In this configuration of the ElectroPrep™, the sample is placed in a Union between two membranes (b), both of which should have a MWCO larger than the molecular weight of the molecule of interest, while the MWCOs of membranes (a) and (c) are smaller. Each biological molecule, including the molecule of interest, will then move either to Dialysis Chamber (1) or Dialysis Chamber (2), depending on its respective charge polarity (i.e. positive or negative), whereas those biological molecules with molecular weights lower than the sample of interest will migrate through membranes (a) and (c) into the tank. Biological molecules with unknown isoelectric points can also be separated and purified in this manner. Dialysis Chambers of smaller volumes can be used to concentrate dialysed product.

(IV) Electrodialysis through Simultaneous Exchange of Buffers - e.g. cleaning up PCR®products
Following amplification, the PCR® product is placed in the Dialysis Chamber between membranes (a) and (b), each membrane having a MWCO lower than the molecular weight of the PCR? product but not the primer. Upon application of the electric field, dialysis occurs quickly by the simultaneous exchange of buffer, resulting in the migration of the lower molecular weight primers through membranes (a) and (b) while the purified PCR? product is retained in the Dialysis Chamber. Electrodialysis is also effective for desalting neutral molecules that do not move in an electric field (such as sugars) or charged molecules at their isoelectric point.



Benefits
Fast dialysis times due to rapid movement of charged molecules in an electric field
Re-usable
Different chambers for large or small sample volumes
Membranes available with MWCOs to suit almost any application or molecule size
Easy to use
Leak proof
Autoclavable
Inert Teflon construction minimises non-specific protein and nucleic acid binding to the sample chamber, enhancing sample recovery
Highly versatile can be adapted for multiple dialysis applications
ApplicationsElectroelution from gels and solutions - e.g. gel extraction of vector and insert during cloning

Electrodialysis (with an average buffer exchange time of 5 to 10 minutes)

On-line electrodialysis

Electroconcentration

Selective electrofiltration

Size-fractionation of DNA and proteins from complex lysates

Primer removal following PCR® amplification

Salt removal from DNA mini-preps

Detergent removal

Dye-Terminator removal


Ordering Information

Unit Only

ElectroPrep™ Tank, including 4mm shrouded power connectorsEP-74-1101677,00 €

Replacement Parts & Accessories

2 x 1-metre power leads with shrouded 4 mm power output connectorsCABLE-453,00 €Add to your cart

Technical Specification
Unit Dimensions (WxLxH)12.5x25x14cm
Internal Dimensions (WxLxH)9.5x20x8cm
Buffer Volume1500ml
Recommended Running Conditions5 to 10mA; 10 minutes run-time
Power Output Connectors (diameter)Shrouded, 4mm
Recommended Power SuppliesConsort EV243
Benefits
Ordering Information
Technical Specifications


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