Chromosome Resolution Additive

Chromosome Resolution Additive

CRA (Chromosome Resolution Additive) is a newly discovered commercial formulation that consistently prevents chromosome contraction and encourages chromosome elongation by loosening the chemical bonding within the supercoiled structure of the chromosome. The result is prophase quality chromosomes. It was developed especially for tissues resistant to existing prophase techniques, such as bone marrows and solid tumors. CRA contains no ethidium bromide or thymidine.

CRA (Chromosome Resolution Additive) is a newly discovered commercial formulation that consistently prevents chromosome contraction and encourages chromosome elongation by loosening the chemical bonding within the supercoiled structure of the chromosome. The result is prophase quality chromosomes. It was developed especially for tissues resistant to existing prophase techniques, such as bone marrows and solid tumors. CRA contains no ethidium bromide or thymidine.

Handling

Dissolve 0.1ml of CRA stock in 9.9ml of injection quality water. Only use 0.1ml of working solution per 10ml culture. Working solution must be used and never stored. NOTE: Scientists can make up smaller working volumes of CRA but should always maintain the above ratio ie. 1 part CRA in 99 parts injection quality water



Applications & Benefits
Counters the 'shrinking' effect of colchicine, acting as a molecular wedge
CRA produces longer chromosomes without breakage
Easier to use and low toxicity compared to Ethidium Bromide
A novel formulation which prevents chemical contraction and encourages chromosome elongation by loosening the bonding within the supercoiled intrachromosomal structure. Antagonistic to the supercontracting effects of colchicine exposure, CRA acts as a molecular wedge, causing the chromosome structure to elongate. CRA is specially developed for tissues resistant to existing prophase techniques, such as bone marrow and tumour samples. This product does not contain Ethidium Bromide or Thymidine. CRA does not induce chromosome breakage.


Ordering Information
Chromosome Resolution Additive - 1 mlGGS-JL003/a88,00 €

Technical Specification

Procedure

1.Add 0.1 ml of CRA (working solution) to each culture containing 10ml of culture medium (see note above).
2.Incubate at 37 °C for 90 mins.
3.Harvest as usual.

Storage

Not for drug or other use
CRA is prepared in phosphate buffered saline sterile, filtered, and supplied in a stock solution consisting of a 1ml vial with a shelf-life of 12 months.
The stock solution should be stored in an air-tight container at 2-6°C.
Never freeze this product.
For Laboratory research use only.
Applications & Benefits
Ordering Information
Technical Specifications






Downloads
PDF-FileProCell_Brochure.pdf
Brochure
0.49 MB
PDF-FileProcell A4 leaflet.pdf
Flyer
1.88 MB
PDF-FileChromResolution_Add_...
MSDS
0.39 MB


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