Buffered Hypotonic Solution
Buffered Hypotonic Solution is a hypotonic solution developed for bone marrow specimens. It has been found to achieve good metaphase swelling (spreading) from a wide range of sample types and significantly increases the number of useful metaphases in leukaemic bone marrow cultures when compared to the standard 0.075M KCL hypotonic. It contains 0.4% KCL with HEPES. 
| Developed for the most challenging samples | | Two products - Optimal and Buffered Hypotonic | | Work on tumour and bone marrow samples or any other samples with very 'tight' metaphases eg keratinocyte, problem blood, rhinoceros(!) etc | | Excellent improvements of cytogenetic stem cell preparations | | Buffered Hypotonic Solution is a hypotonic solution developed for bone marrow specimens. It has been found to achieve good metaphase swelling (spreading) from a wide range of sample types and significantly increases the number of useful metaphases in leukaemic bone marrow cultures when compared to the standard 0.075M KCL hypotonic. It contains 0.4% KCL with HEPES. |
| Buffered Hypotonic Solution - Powder for 250 ml solution | GGS-JL006/a | 48,00 € |
| Buffered Hypotonic Solution - Powder for 1000 ml solution | GGS-JL006/b | 149,00 € |
Proceduresee ordering details | | 1. | Add the powdered contents of the supplied bottle of Buffered Hypotonic Solution product to 1000 mls of distilled water. | | 2. | Adjust to pH 7.4 with 5N NaOH and store at 4°C in a clean glass bottle, until ready to use, noting down the date on the bottle. | | 3. | During harvest replace your existing hypotonic solution with Buffered Hypotonic Solution | | 4. | Replace your existing hypotonic and incubate cells at 37°C for a maximum of 30 minutes. |
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