OD600 DiluPhotometer™

OD600 DiluPhotometer™

Easy to use and small

The OD600 DiluPhotometer™ is a small, easy to use instrument that is dedicated to measuring samples near the 600 nm wavelength. It is suitable for measuring growth rates of several cell types including E. coli and yeast. Use of the OD600 DiluPhotometer™ with Implen?s DiluCell™ 10 or DiluCell™ 20 facilitates the measurement of low volume samples (volume requirement of 200 ?l and 100 ?l respectively). The two different available DiluCell™ 10 and DiluCell™ 20 versions allow for automatic 1/10 and 1/20 dilutions. With a bandwidth of 40 nm, the OD600 is also suitable for protein quantification assays like Bradford (595 nm). The small size and portable nature of the OD600 DiluPhotometer™ make it convenient for use in incubation cabinets and under anaerobic conditions.

The OD600 DiluPhotometer™ has been designed for the spectrophotometric analysis of bacterial and yeast growth rates and the quantification of proteins at a wavelength nearby 600 nm (e.g. Bradford). The instrument is battery operated and can be easily transported for usage in incubation cabinets and under anaerobic conditions. The batteries last for almost 1 month when fully charged. A 600nm LED source in combination with a fiber optic is used to obtain the optical density measurement. Spills can be easily wiped from the surface, and the cell compartment area can be rinsed with, formaldehyde or ethylene oxide to sterilize it.

The OD600 DiluPhotometer™ has been designed for the spectrophotometric analysis of bacterial and yeast growth rates and the quantification of proteins at a wavelength nearby 600 nm (e.g. Bradford). The instrument is battery operated and can be easily transported for usage in incubation cabinets and under anaerobic conditions. The batteries last for almost 1 month when fully charged. A 600nm LED source in combination with a fiber optic is used to obtain the optical density measurement. Spills can be easily wiped from the surface, and the cell compartment area can be rinsed with, formaldehyde or ethylene oxide to sterilize it.

Lambert-Beer-Law

The Lambert-Beer-Law, also known as Beer?s Law, empirically relates the absorption of light to the properties of the sample. This law states that there is a logarithmic relationship between the transmission of light through a specific sample (T = I/Io with I = outgoing light and Io = incoming light), the molar extinction coefficient for a specific compound (?), the concentration of the absorbing species in the material (c) and the distance the light travels (d). By leaving all conditions constant but changing the pathlength (distance the light travels), you can bend the Beer-Law to get an automatic dilution of your sample. This principle is realized within the OD600 DiluPhotometer™ with DiluCell™.

The Lambert-Beer-Law, also known as Beer?s Law, empirically relates the absorption of light to the properties of the sample. This law states that there is a logarithmic relationship between the transmission of light through a specific sample (T = I/Io with I = outgoing light and Io = incoming light), the molar extinction coefficient for a specific compound (?), the concentration of the absorbing species in the material (c) and the distance the light travels (d). By leaving all conditions constant but changing the pathlength (distance the light travels), you can bend the Beer-Law to get an automatic dilution of your sample. This principle is realized within the OD600 DiluPhotometer™ with DiluCell™.

Automatic Sample Dilution

Previously it was necessary to use standard cuvettes with a volume of at least 1 ml and additionally to dilute the sample manually. DiluCell™ from Implen allows for lower sample volume requirements and provides automatic virtual sample dilution. Due to the unique design of DiluCell™ cuvettes, the light-path is reduced from the standard 10 mm to 0.5 mm (DC 20) or 1.0 mm (DC 10). According to the Lambert Beer Law this shortened pathlength results in an automatic sample dilution by factor 20 (DC 20) or 10 (DC 10). Automatic dilution with DiluCell™ saves time and excludes dilution errors and cross contaminations.

Previously it was necessary to use standard cuvettes with a volume of at least 1 ml and additionally to dilute the sample manually. DiluCell™ from Implen allows for lower sample volume requirements and provides automatic virtual sample dilution. Due to the unique design of DiluCell™ cuvettes, the light-path is reduced from the standard 10 mm to 0.5 mm (DC 20) or 1.0 mm (DC 10). According to the Lambert Beer Law this shortened pathlength results in an automatic sample dilution by factor 20 (DC 20) or 10 (DC 10). Automatic dilution with DiluCell™ saves time and excludes dilution errors and cross contaminations.

Measuring Cell Density

The stage of growth of a bacterial culture needs to be monitored to ensure that the cells are harvested at the optimum point (towards the end of log phase) for the greatest density of live cells. The optical density of the sample indicates when this point has been reached. This value varies dependent on the cells being grown. Routinely cells are grown until the absorbance at 600 nm, also known as OD600, reaches approximately 0.4 prior to induction or harvesting. A linear relationship exists between cell number (density) and OD 600 up to approx. 0.6.

The stage of growth of a bacterial culture needs to be monitored to ensure that the cells are harvested at the optimum point (towards the end of log phase) for the greatest density of live cells. The optical density of the sample indicates when this point has been reached. This value varies dependent on the cells being grown. Routinely cells are grown until the absorbance at 600 nm, also known as OD600, reaches approximately 0.4 prior to induction or harvesting. A linear relationship exists between cell number (density) and OD 600 up to approx. 0.6.



Applications & Benefits
Small and portable - DiluPhotometer™ for fast quantification.
DiluCell™ - Dilute cells automatically
OD600 Values - Fast and easy determination of cell density at 600 nm.
Bradford Assay - Quantify protein with Bradford protein assay
Battery Powered - Simple to charge and take with you anywhere
Budget friendly


Ordering Information
OD600 DiluPhotometer™ for the determination of cell density and Bradford assays (590 - 610 nm), battery powered, RS232 port for data transfer, charger, cables and manual, incl. DiluCell™ 20 ? disposable cuvette for spectrophotometrical analysis of protein samples or microbial cell cultures, necessary sample volume: 100 ?l, virtual dilution 1/20, path length 0.5 mm, center height between 8.5 mm and 15 mm, 4 packs of 96 piecesOD-600-101990,00 €
OD600 DiluPhotometer™ for the determination of cell density and Bradford assays (590 - 610 nm), battery powered, RS232 port for data transfer, charger, cables and manual, incl. DiluCell™ 10 ? disposable cuvette for spectrophotometrical analysis of protein samples or microbial cell cultures, necessary sample volume: 200 ?l, virtual dilution 1/10, path length 1 mm, center height between 8.5 mm and 15 mm, 2 packs of 96 pieces and DiluCell™ 20 ? disposable cuvette for spectrophotometrical analysis of protein samples or microbial cell cultures, necessary sample volume: 100 ?l, virtual dilution 1/20, path length 0.5 mm, center height between 8.5 mm and 15 mm, 2 packs of 96 piecesOD-600-10201990,00 €
DiluCell™ 10 - disposable cuvette for spectrophotometrical analysis of protein samples or microbial cell cultures, necessary sample volume: 200 µl, virtual dilution 1/10, path length 1.0 mm, center height between 8.5 mm and 15 mm, pack of 96DC109695,00 €
DiluCell™ 20 - disposable cuvette for spectrophotometrical analysis of protein samples or microbial cell cultures, necessary sample volume: 100 µl, virtual dilution 1/20, path length 0.5 mm, center height between 8.5 mm and 15 mm, pack of 96DC209695,00 €

Technical Specification
Wavelength600nm
Bandwidth40nm
RangeOptical Density ? 0.3A to 1.99A
RangeOptical Density ? 0.3A to 1.99A
Accuracy< ±0.05A at 1A using Neutral Density Filters
Repeatability±0.02A at 1A
Controls6 button ? power, blank, sample, memory, re-set, recall/print
Optical Height8.5mm
Cuvette HolderFixed with drain hole. Accepts DiluCell™ 10, DiluCell™ 20, 10 mm pathlength semi micro and macro cuvettes or 14 ? 16 mm round tubes.
OutputRS232, USB
Memory99 readings
DisplayCustom LCD
Power RequirementsExternal power adaptor (110 to 220 V, 50/60 Hz, 20 VA) or internal rechargeable NiMH battery
Approximate Dimensions180 x 150 x 60mm
Weight0.6kg
Applications & Benefits
Ordering Information
Technical Specifications






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