Pre-Hypotonic Solution

Pre-Hypotonic Solutionis to be used before the hypotonic stage of a blood or bone marrow harvest. Pre-hypotonic Solution makes the hypotonic step more effective by circumventing the tendency of chromosomes to clump or to under-spread. The clumping of metaphases during the harvesting procedure often results in the loss of useful metaphases or the loss of entire cultures. Procedures such as FISH that require good well spread metaphases are often hampered by this problem. Pre-Hypotonic Solution was developed to address this clumping problem. Optimal metaphase swelling can therefore subsequently be achieved by your hypotonic solution.

Applications & Benefits

Powerful Hypotonic Solutions
Developed for the most challenging samples
Two products - Optimal and Buffered Hypotonic
Work on tumour and bone marrow samples or any other samples with very 'tight' metaphases eg keratinocyte, problem blood, rhinoceros(!) etc
Excellent improvements of cytogenetic stem cell preparations
Pre-Hypotonic Solution was developed to address this clumping problem. Optimal metaphase swelling can therefore subsequently be achieved by your hypotonic solution. Pre-Hypotonic Solution has been found to be particularly useful for template CGH metaphases. CGH hybridisations have been improved by Pre-Hypotonic Solution.

Ordering Information

Prehypotonic Solution - 5ml

GGS-JL007

132,00 €

Technical Specification


Procedure 1
1.	After colchicine/colcemid/Metaphase Arresting Solution incubation is complete, spin down the culture discarding the supernatant.
2.	Mix the cell pellet well and add 10 ml of culture media, followed by 1 ml of Pre Hypotonic Solution. Mix well.
3.	Centrifuge for 10 minutes at 1000 rpm
4.	Discard the supernatant; proceed with the harvest as normal from, and including, the hypotonic step.


Procedure 2
1.	Pipette out the old medium.
2.	Add 10mls of fresh medium, followed by 1ml of prehypotonic.
3.	Wait for 10 minutes.
4.	Pipette out the prehypotonic.
5.	Harvest as usual


Pre Hypotonic Solution Preparation
1.	Add the contents of the supplied 5 ml vial to a 1000 ml volumetric flask
2.	Bring to 1000ml with milli-Q water or reverse osmosis-filtered deionised water