Cytoclear
Cytoclear is a newly developed product (consisting of a mixture of protein detergents) which can quickly dissolve and remove persistent cytoplasm (mainly caused by inadequate hypotonic treatment) during the methanol:acetic acid fixation stage of the chromosome harvesting procedure. In particular, it dissolves the lipid and glycoprotein structure of which cytoplasm is mainly composed. Clumped metaphases, which would normally be impossible to analyse, can now be restored
| For cultures that have yielded poor quality metaphases. | | Effective cytoplasm removal | | Improves metaphase quality | | Produces brighter FISH signals | | Can be used to rescue bad harvests |
| Cytoclear - 1 ml | GGS-JL004 | 220,00 € |
| Procedure 1 | | 1. | At the first fix step in the harvest, add 5ul of Cytoclear per ml of methanol: acetic acid (normally 3:1 ratio or whatever is the laboratory standard | | 2. | Stand for 5 minutes | | 3. | Centrifuge for 5 minutes at 1000rpm | | 4. | Continue the harvest as normal. |
| | Procedure 2
For cultures that have yielded poor quality metaphases | | 1. | Identify problem cultures by checking freshly made slides under phase contrast | | 2. | Take the problem culture, add 5ul of Cytoclear per ml of methanol: acetic acid (normally 3:1 ratio or whatever is the laboratory standard ratio), as n | | 3. | Stand at RT for 10 minutes. | | 4. | Spin down, then refix. | | 5. | Make slides |
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