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Eve – Automatic trypan blue cell counter

Technology

The EVE™ automatic cell counter uses state-of-the-art optics and image analysis for automatic cell counting. It is a benchtop counter designed to measure cell count and viability (live, dead, and total cells) accurately and precisely, using the standard trypan blue… ⇒ technique. Using the same amount of sample that you currently use with the hemocytometer, the system takes less than 20 seconds per sample for a typical cell count and is compatible with a wide variety of eukaryotic cells and provides information on cell size

Description

Procedure

A single, sample measurement in less than 30 seconds produces the following results:

  • Live and dead cell concentration/mL
  • Total cell concentration/mL
  • Viability (% live cells to total cells)
  • Mean diameter
  • Cell images
  • Graphical data representation

Trypan blue staining

Trypan blue is a vital diazo dye stain used to selectively colour dead tissues or cells blue. Live cells or tissues with intact cell membranes are not coloured. Since cells are very selective in the compounds that pass through the membrane, in a viable cell trypan blue is not absorbed; however, it traverses the membrane in a dead cell.

Hence, dead cells are shown as a distinctive blue colour under a microscope. Since live cells are excluded from staining, this staining method is also described as a dye exclusion method.

EVE™ is supplied with disposable cell counting chamber slides that contain two enclosed chambers to hold the sample to allow you to measure two different samples or perform replicates of the same sample. The cell counting occurs in the central location of the counting chamber and the volume counted is 0.4µL, the same as counting four (1 mm x 1 mm) squares in a standard hemocytometer

Software description

Comparison image (Clumped cells)
Competitor's automated cell counter
The MCF-7 breast cancer cell line can be clumpy.The EVE counting algorithm identifies and counts individual cells within these cell clumps for accurate analysis.
Correlation of EVETM and manual counting
Measuring from the EVE™ extend further along the high concentration range than hemocytometer readings.
User friendly
Self explaining easy to use interface.

Cell lines validated on EVE

Cell Type AnimalOrganGrowth Properties
HeLa Human Skin Adherent
NIH-3T3 Mouse Embryo Adherent
U2-OS Human Bone Adherent
Jurkat Human Blood Suspension
KG-1 Human Blood Suspension
HepG2 Human Liver Adherent
Hep3B Human Liver Adherent
LNCaP Human Prostate Adherent
SH-SY5Y Human Brain Adherent
SCN2.2 Rat Brain Adherent
F9 Mouse Embryo Adherent
MCF7 Human Breast Adherent
A549 Human Lung Adherent
GH3 Rat Pituitary gland Adherent

Applications & Benefits

Focused design

Designed to measure cell count and viability (live, dead, and total cells) accurately and precisely, using the standard trypan blue technique.

Speed

Using the same amount of sample that is currently used with the hemocytometer, the EVE™ takes less than 20 seconds per sample for a typical cell count

Usability

Provides data for broad range of cell sizes and types. Primary (tissue, blood) cell lines and stem cells. Measures cell concentrations ranging from 1 x 104 to 1 x 107 cells/mL and cells with sizes ranging from 5 µm to 60 µm

User friendlyness

Intuitive user interface. 7 inch LCD touch screen, Bench top size, No maintenance needed.

Reproducibility and sensitivity

The cell counting occurs in the central location of the counting chamber and the volume counted is 0.4 µL, the same as counting four (1 mm x 1 mm) squares in a standard hemocytometer

No cross contamination

Uses disposable cell counting slides that eliminate washing steps and cross contamination between samples.

Data storage and analysis

Stores 500 test results, transfer the data to PC using USB drive. PC based software provided for in-depth analysis. Presents comprehensive data with graphical reports and as a . CSV(comma separated value) file for sample comparisons

Differentiation of clumped cells

Provides the clumping cell counting function to get more accurate counting results with an advanced analysis algorithm in clumped cell

Cell size gating

Select range of cell sizes using gating function

Technical Specification

Instrument Specifications
Counting time< 20 seconds
Cell measurement range (cells/mL)1 x 104 - 1 x 107 cells/ml
Optimal measurement range (cells/mL)1 X 105 - 4 x 106 cells/ml
Cell size range50-60 µm
Sample volume10 µL
Staining MethodTrypan blue stain
Display7″ LCD touch screen
Image formatJPEG (image), CSV (raw data)
Data exportUSB drive
Dimensions27 cm (w) x 20 cm (d) x 19 cm (h)
Weight2.1 kg
Operating Power100-240 VAC, 1.5A
Frequency50/60 Hz
Electrical Input12 VDC, 1.0 A
Operating Temperature10-35°C
Maximum Relative Humidity20-80%
Altitude< 2000 m
Cell Counting Chamber Slide Specifications
Chamber Volume10 µL
MaterialPolymethylmethacrylate (PMMA)
Dimensions75 mm (w) x 25 mm (d) x 1.8 mm (h)
Chamber Depth100 µm

Ordering Information

Complete System
EVE™ Automatic Cell Counter, Main Device, Power Supply, USB Drive 50 Cell Counting Slides ( 100 counts) with 1x 1,5ml Trypan Blue 0,4%EVE-MC 2.190,00 €
Parts & Accessories
50 Cell Counting Slides ( 100 counts) with 1x 1,5ml Trypan Blue 0,4%EVS-005079,00 €
500 Cell Counting Slides ( 1.000 counts ), with 10 x 1,5ml Trypan Blue 0,4%EVS-0500685,00 €
1.000 Cell Counting Slides ( 2.000 counts ), with 20 x 1,5ml Trypan Blue 0,4%EVS-10001.180,00 €
2,500 Cell Counting Slides ( 5.000 counts ), with 100 x 1,5ml Trypan Blue 0,4%EVS-25002.730,00 €
5.000 Cell Counting Slides ( 10.000 counts ), with 100 x 1,5ml Trypan Blue 0,4%EVS-50005.250,00 €
Test Beads. Concentration (avg.) 1.0 X 106EBB-00190,00 €
2ml Trypan Blue Stain 0,4%, 2X1 ml For the use with the Countess® Cell Counter.
Substitutes Countess® Order Number T10282		TB-0225,00 €
Parts & Accessories compatible with Countess® Cell Counter
50 Cell Counting Slides ( 100 counts). For the use with the Countess® Cell Counter.
Substitutes Countess® Order Number C10228		CEVS-005079,00 €
500 Cell Counting Slides ( 1.000 counts ) For the use with the Countess® Cell Counter.
Substitutes Countess® Order Number C10312		CEVS-0500685,00 €
1.250 Cell Counting Slides ( 2.000 counts ) For the use with the Countess® Cell Counter.
Substitutes Countess® Order Number C10313		CEVS-12501.438,00 €
2.500 Cell Counting Slides (10.000 counts) For the use with the Countess® Cell Counter.
Substitutes Countess® Order Number C10314		CEVS-25002.731,00 €
5.000 Cell Counting Slides ( 10.000 counts ) For the use with the Countess® Cell Counter.
Substitutes Countess® Order Number C10315		CEVS-50005.250,00 €
Test Beads. Concentration (avg.) 1.0 X 106EBB-00190,00 €
2ml Trypan Blue Stain 0,4%, 2X1 ml For the use with the Countess® Cell Counter.
Substitutes Countess® Order Number T10282		TB-0225,00 €
20 ml Trypan Blue Stain 0,4%, 20 X1 ml. For the use with the Countess® Cell Counter.
Substitutes Countess® Order Number T10282		TB-20170,00 €

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